| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Bell, Fina M (12)
|
NITTSC |
31.24 |
31.23 |
-0.01 |
| 2 |
Hesch, Natalie G (12)
|
NITTSC |
31.10 |
32.01 |
0.91 |
| 3 |
Mutty, Vivian M (11)
|
NIEAST |
34.24 |
34.53 |
0.29 |
| 4 |
Musterait, Mallory E (12)
|
NITWST |
39.00 |
36.65 |
-2.35 |
| 5 |
Sauer, Emma L (11)
|
NIHAMB |
36.37 |
36.95 |
0.58 |
| 6 |
Latona, Dena M (12)
|
NIHAMB |
34.72 |
37.12 |
2.40 |
| 7 |
Zolet, Marta E (12)
|
NIEAST |
37.07 |
37.22 |
0.15 |
| 8 |
Dahl, Sydney R (12)
|
NIPVSC |
39.40 |
37.70 |
-1.70 |
| 9 |
Rydzewski, Gabriella D (11)
|
NITWST |
42.68 |
38.74 |
-3.94 |
| 10 |
Hartman, Shea A (11)
|
NITTSC |
|
40.16 |
- |
| 11 |
Hann, Hailey E (11)
|
NICSCI |
45.41 |
41.16 |
-4.25 |
| 12 |
Jones, Sophia M (11)
|
NIPVSC |
47.15 |
47.39 |
0.24 |
|
Bauman, Cameryn (11)
|
NIEAST |
50.73 |
DQ |
- |
|
Hammond, Taylor M (12)
|
NIEMAC |
|
DQ |
- |
|
Marcyan, Maya G (12)
|
NITTSC |
31.40 |
DQ |
- |
|
Mulawka, Paige M (12)
|
NIHAMB |
34.75 |
NS |
- |